Detailed information of TA system TA042028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54810..55360
• Replicon: chromosome
• Accession: NZ_AP026095
• Organism: Escherichia coli strain 53_142304

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: OO816_RS00250
• Protein ID: WP_001160975.1
• Coordinates: 55046..55360 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: OO816_RS00245
• Protein ID: WP_000125566.1
• Coordinates: 54810..55043 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO816_RS00220 (49884)	49884..50171	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
OO816_RS00225 (50229)	50229..51560	+	1332	WP_001183177.1	MFS transporter	-
OO816_RS00230 (51668)	51668..52198	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
OO816_RS00235 (52191)	52191..54053	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
OO816_RS00240 (54245)	54245..54724	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
OO816_RS00245 (54810)	54810..55043	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
OO816_RS00250 (55046)	55046..55360	+	315	WP_001160975.1	CcdB family protein	Toxin
OO816_RS00255 (55357)	55357..56205	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
OO816_RS00260 (56212)	56212..56589	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
OO816_RS00265 (56592)	56592..57413	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
OO816_RS00270 (57410)	57410..58399	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
OO816_RS00275 (58399)	58399..59685	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T42028 WP_001160975.1 NZ_AP026095:55046-55360 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T42028 NZ_AP026095:55046-55360 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT42028 WP_000125566.1 NZ_AP026095:54810-55043 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT42028 NZ_AP026095:54810-55043 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9