Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1838..2385 Replicon plasmid pNIID27_2
Accession NZ_AP026094
Organism Escherichia coli strain 27_141091

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag OO959_RS27410 Protein ID WP_001384452.1
Coordinates 2107..2385 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag OO959_RS27405 Protein ID WP_000079928.1
Coordinates 1838..2107 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO959_RS27390 (1) 1..75 + 75 WP_001365705.1 RepA leader peptide Tap -
OO959_RS27395 (68) 68..925 + 858 WP_001554370.1 incFII family plasmid replication initiator RepA -
OO959_RS27400 (1625) 1625..1783 + 159 WP_192795837.1 hypothetical protein -
OO959_RS27405 (1838) 1838..2107 + 270 WP_000079928.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
OO959_RS27410 (2107) 2107..2385 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
OO959_RS27415 (2470) 2470..2817 + 348 WP_000142452.1 DUF6404 family protein -
OO959_RS27420 (2835) 2835..3425 - 591 WP_042004720.1 hypothetical protein -
OO959_RS27425 (3422) 3422..3682 - 261 WP_019842588.1 hypothetical protein -
OO959_RS27430 (4251) 4251..4664 + 414 WP_000465034.1 hypothetical protein -
OO959_RS27435 (4666) 4666..5448 + 783 WP_001164199.1 site-specific integrase -
OO959_RS27440 (5621) 5621..5974 + 354 WP_001401712.1 colicin M immunity protein -
OO959_RS27445 (6024) 6024..6839 - 816 WP_000449473.1 lipid II-degrading bacteriocin colicin M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..66944 66944


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T42024 WP_001384452.1 NZ_AP026094:2107-2385 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T42024 NZ_AP026094:2107-2385 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTAGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT42024 WP_000079928.1 NZ_AP026094:1838-2107 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT42024 NZ_AP026094:1838-2107 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References