Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51863..52388 Replicon plasmid pNIID27_1
Accession NZ_AP026093
Organism Escherichia coli strain 27_141091

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag OO959_RS27165 Protein ID WP_001159868.1
Coordinates 52083..52388 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag OO959_RS27160 Protein ID WP_000813634.1
Coordinates 51863..52081 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO959_RS27130 (47146) 47146..47376 + 231 WP_010891288.1 hypothetical protein -
OO959_RS27135 (47497) 47497..48237 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
OO959_RS27140 (48522) 48522..49499 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
OO959_RS27145 (49907) 49907..50107 - 201 WP_000708307.1 hypothetical protein -
OO959_RS27150 (50104) 50104..50724 - 621 WP_001248529.1 hypothetical protein -
OO959_RS27155 (50721) 50721..51404 - 684 WP_010891291.1 DNA-binding protein -
OO959_RS27160 (51863) 51863..52081 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
OO959_RS27165 (52083) 52083..52388 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
OO959_RS27170 (52389) 52389..53195 + 807 WP_000016989.1 site-specific integrase -
OO959_RS27175 (53311) 53311..53673 + 363 Protein_54 RepB family plasmid replication initiator protein -
OO959_RS27180 (54261) 54261..55427 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -
OO959_RS27185 (55427) 55427..56398 + 972 WP_000817031.1 ParB/RepB/Spo0J family plasmid partition protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..91003 91003


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T42023 WP_001159868.1 NZ_AP026093:52083-52388 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T42023 NZ_AP026093:52083-52388 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT42023 WP_000813634.1 NZ_AP026093:51863-52081 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT42023 NZ_AP026093:51863-52081 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References