Detailed information of TA system TA042017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5231261..5231856
• Replicon: chromosome
• Accession: NZ_AP026092
• Organism: Escherichia coli strain 27_141091

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: OO959_RS26100
• Protein ID: WP_000239579.1
• Coordinates: 5231506..5231856 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: OO959_RS26095
• Protein ID: WP_001223210.1
• Coordinates: 5231261..5231512 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO959_RS26085 (5226926)	5226926..5230705	+	3780	WP_264832281.1	autotransporter assembly complex protein TamB	-
OO959_RS26090 (5230708)	5230708..5231049	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
OO959_RS26095 (5231261)	5231261..5231512	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
OO959_RS26100 (5231506)	5231506..5231856	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
OO959_RS26105 (5231936)	5231936..5232466	-	531	WP_000055075.1	inorganic diphosphatase	-
OO959_RS26110 (5232776)	5232776..5233732	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
OO959_RS26115 (5233872)	5233872..5235374	+	1503	Protein_5101	sugar ABC transporter ATP-binding protein	-
OO959_RS26120 (5235388)	5235388..5236410	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T42017 WP_000239579.1 NZ_AP026092:5231506-5231856 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T42017 NZ_AP026092:5231506-5231856 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT42017 WP_001223210.1 NZ_AP026092:5231261-5231512 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT42017 NZ_AP026092:5231261-5231512 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8