Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5134385..5134882 Replicon chromosome
Accession NZ_AP026092
Organism Escherichia coli strain 27_141091

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO959_RS25600 Protein ID WP_001173343.1
Coordinates 5134709..5134882 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO959_RS25595 Protein ID WP_000398619.1
Coordinates 5134385..5134681 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO959_RS25575 (5131116) 5131116..5131835 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO959_RS25580 (5131832) 5131832..5133463 - 1632 WP_001216477.1 sensor histidine kinase -
OO959_RS25585 (5133644) 5133644..5133874 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO959_RS25590 (5133886) 5133886..5134158 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO959_RS25595 (5134385) 5134385..5134681 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO959_RS25600 (5134709) 5134709..5134882 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO959_RS25605 (5135001) 5135001..5136518 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO959_RS25610 (5136755) 5136755..5138212 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42016 WP_001173343.1 NZ_AP026092:5134709-5134882 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42016 NZ_AP026092:5134709-5134882 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42016 WP_000398619.1 NZ_AP026092:5134385-5134681 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42016 NZ_AP026092:5134385-5134681 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References