Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5140723..5141220 Replicon chromosome
Accession NZ_AP026090
Organism Escherichia coli strain 26_141088

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO788_RS25735 Protein ID WP_001173343.1
Coordinates 5141047..5141220 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO788_RS25730 Protein ID WP_000398619.1
Coordinates 5140723..5141019 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO788_RS25710 (5137454) 5137454..5138173 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO788_RS25715 (5138170) 5138170..5139801 - 1632 WP_001216477.1 sensor histidine kinase -
OO788_RS25720 (5139982) 5139982..5140212 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO788_RS25725 (5140224) 5140224..5140496 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO788_RS25730 (5140723) 5140723..5141019 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO788_RS25735 (5141047) 5141047..5141220 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO788_RS25740 (5141339) 5141339..5142856 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO788_RS25745 (5143093) 5143093..5144550 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T41980 WP_001173343.1 NZ_AP026090:5141047-5141220 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T41980 NZ_AP026090:5141047-5141220 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT41980 WP_000398619.1 NZ_AP026090:5140723-5141019 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT41980 NZ_AP026090:5140723-5141019 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References