Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 30800..31444 Replicon plasmid pNIID8_2
Accession NZ_AP026089
Organism Escherichia coli strain 8_140198

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag OO841_RS28095 Protein ID WP_000833471.1
Coordinates 31262..31444 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag OO841_RS28090 Protein ID WP_000466317.1
Coordinates 30800..31237 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO841_RS28070 (CL8NIID8_53860) 28210..28545 - 336 WP_000963832.1 DUF6290 family protein -
OO841_RS28075 (CL8NIID8_53880) 28892..29299 + 408 WP_001190621.1 hypothetical protein -
OO841_RS28080 (CL8NIID8_53890) 29309..30070 + 762 WP_001097938.1 conjugal transfer protein TraL -
OO841_RS28085 (CL8NIID8_53900) 30067..30771 + 705 WP_001279862.1 hypothetical protein -
OO841_RS28090 (CL8NIID8_53910) 30800..31237 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO841_RS28095 31262..31444 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
OO841_RS28100 (CL8NIID8_53920) 31879..32157 - 279 WP_000545933.1 hypothetical protein -
OO841_RS28105 (CL8NIID8_53930) 32215..32544 - 330 WP_000855123.1 hypothetical protein -
OO841_RS28110 (CL8NIID8_53940) 32983..33225 - 243 WP_001479502.1 transcriptional regulator -
OO841_RS28115 (CL8NIID8_53950) 33215..33481 - 267 WP_000868277.1 hypothetical protein -
OO841_RS28120 (CL8NIID8_53960) 33532..33756 - 225 WP_000749828.1 hypothetical protein -
OO841_RS28125 (CL8NIID8_53970) 33852..34076 - 225 WP_000866040.1 hypothetical protein -
OO841_RS28130 (CL8NIID8_53980) 34116..34439 - 324 WP_000432877.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..36062 36062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T41948 WP_000833471.1 NZ_AP026089:c31444-31262 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T41948 NZ_AP026089:c31444-31262 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT41948 WP_000466317.1 NZ_AP026089:c31237-30800 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT41948 NZ_AP026089:c31237-30800 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References