Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 2328..2887 Replicon plasmid pNIID8_1
Accession NZ_AP026088
Organism Escherichia coli strain 8_140198

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag OO841_RS27465 Protein ID WP_000421248.1
Coordinates 2612..2887 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag OO841_RS27460 Protein ID WP_001178089.1
Coordinates 2328..2612 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO841_RS27445 (1) 1..255 + 255 WP_000083831.1 replication regulatory protein RepA -
OO841_RS27450 (491) 491..565 + 75 WP_001370046.1 RepA leader peptide Tap -
OO841_RS27455 (558) 558..1415 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
OO841_RS27460 (2328) 2328..2612 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
OO841_RS27465 (2612) 2612..2887 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO841_RS27470 (2982) 2982..3188 + 207 WP_001105064.1 hypothetical protein -
OO841_RS27475 (3288) 3288..3503 - 216 Protein_6 transposase -
OO841_RS27480 (3547) 3547..4113 - 567 Protein_7 IS3-like element IS600 family transposase -
OO841_RS27485 (4113) 4113..4529 - 417 Protein_8 IS91 family transposase -
OO841_RS27490 (4529) 4529..4714 - 186 WP_001302179.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92794 92794


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T41947 WP_000421248.1 NZ_AP026088:2612-2887 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T41947 NZ_AP026088:2612-2887 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT41947 WP_001178089.1 NZ_AP026088:2328-2612 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT41947 NZ_AP026088:2328-2612 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References