Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4367710..4368341 Replicon chromosome
Accession NZ_AP026087
Organism Escherichia coli strain 8_140198

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO841_RS22290 Protein ID WP_001259385.1
Coordinates 4368066..4368341 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO841_RS22285 Protein ID WP_000593555.1
Coordinates 4367710..4368069 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO841_RS22255 (4362822) 4362822..4363295 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO841_RS22260 (4363292) 4363292..4363573 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO841_RS22265 (4363650) 4363650..4365008 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO841_RS22270 (4365001) 4365001..4366509 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO841_RS22275 (4366499) 4366499..4366768 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO841_RS22280 (4366800) 4366800..4367660 + 861 WP_001131788.1 class II aldolase -
OO841_RS22285 (4367710) 4367710..4368069 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO841_RS22290 (4368066) 4368066..4368341 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO841_RS22295 (4368414) 4368414..4369538 - 1125 WP_001301659.1 ABC transporter permease -
OO841_RS22300 (4369538) 4369538..4372273 - 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
OO841_RS22305 (4372270) 4372270..4373337 - 1068 WP_086173773.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T41938 WP_001259385.1 NZ_AP026087:c4368341-4368066 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T41938 NZ_AP026087:c4368341-4368066 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT41938 WP_000593555.1 NZ_AP026087:c4368069-4367710 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT41938 NZ_AP026087:c4368069-4367710 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References