Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 541113..541731 Replicon chromosome
Accession NZ_AP026087
Organism Escherichia coli strain 8_140198

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OO841_RS02545 Protein ID WP_001291435.1
Coordinates 541113..541331 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OO841_RS02550 Protein ID WP_000344800.1
Coordinates 541357..541731 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO841_RS02510 (536400) 536400..536972 + 573 WP_000779831.1 YbaY family lipoprotein -
OO841_RS02515 (537005) 537005..537316 - 312 WP_000409911.1 MGMT family protein -
OO841_RS02525 (537695) 537695..538048 + 354 WP_000878140.1 DUF1428 domain-containing protein -
OO841_RS02530 (538090) 538090..539640 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OO841_RS02535 (539804) 539804..540274 - 471 WP_000136192.1 YlaC family protein -
OO841_RS02540 (540390) 540390..540941 - 552 WP_000102553.1 maltose O-acetyltransferase -
OO841_RS02545 (541113) 541113..541331 - 219 WP_001291435.1 HHA domain-containing protein Toxin
OO841_RS02550 (541357) 541357..541731 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OO841_RS02555 (542277) 542277..545426 - 3150 WP_001132452.1 efflux RND transporter permease AcrB -
OO841_RS02560 (545449) 545449..546642 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T41910 WP_001291435.1 NZ_AP026087:c541331-541113 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T41910 NZ_AP026087:c541331-541113 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT41910 WP_000344800.1 NZ_AP026087:c541731-541357 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT41910 NZ_AP026087:c541731-541357 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References