Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4301872..4302503 Replicon chromosome
Accession NZ_AP026085
Organism Escherichia coli strain H52_982342

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO840_RS21695 Protein ID WP_001259385.1
Coordinates 4302228..4302503 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO840_RS21690 Protein ID WP_000593555.1
Coordinates 4301872..4302231 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO840_RS21660 (4296984) 4296984..4297457 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO840_RS21665 (4297454) 4297454..4297735 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO840_RS21670 (4297812) 4297812..4299170 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO840_RS21675 (4299163) 4299163..4300671 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO840_RS21680 (4300661) 4300661..4300930 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO840_RS21685 (4300962) 4300962..4301822 + 861 WP_001131788.1 class II aldolase -
OO840_RS21690 (4301872) 4301872..4302231 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO840_RS21695 (4302228) 4302228..4302503 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO840_RS21700 (4302576) 4302576..4303700 - 1125 WP_001301659.1 ABC transporter permease -
OO840_RS21705 (4303700) 4303700..4306435 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO840_RS21710 (4306432) 4306432..4307499 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T41897 WP_001259385.1 NZ_AP026085:c4302503-4302228 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T41897 NZ_AP026085:c4302503-4302228 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT41897 WP_000593555.1 NZ_AP026085:c4302231-4301872 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT41897 NZ_AP026085:c4302231-4301872 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References