Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4345486..4346117 Replicon chromosome
Accession NZ_AP026082
Organism Escherichia coli strain F765

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO934_RS22020 Protein ID WP_001259385.1
Coordinates 4345842..4346117 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO934_RS22015 Protein ID WP_000593555.1
Coordinates 4345486..4345845 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO934_RS21985 (4340598) 4340598..4341071 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO934_RS21990 (4341068) 4341068..4341349 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO934_RS21995 (4341426) 4341426..4342784 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO934_RS22000 (4342777) 4342777..4344285 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO934_RS22005 (4344275) 4344275..4344544 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO934_RS22010 (4344576) 4344576..4345436 + 861 WP_001131788.1 class II aldolase -
OO934_RS22015 (4345486) 4345486..4345845 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO934_RS22020 (4345842) 4345842..4346117 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO934_RS22025 (4346190) 4346190..4347314 - 1125 WP_001301659.1 ABC transporter permease -
OO934_RS22030 (4347314) 4347314..4350049 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO934_RS22035 (4350046) 4350046..4351113 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T41862 WP_001259385.1 NZ_AP026082:c4346117-4345842 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T41862 NZ_AP026082:c4346117-4345842 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT41862 WP_000593555.1 NZ_AP026082:c4345845-4345486 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT41862 NZ_AP026082:c4345845-4345486 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References