Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4344268..4344899 Replicon chromosome
Accession NZ_AP026080
Organism Escherichia coli strain F690

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag OO857_RS21955 Protein ID WP_001259385.1
Coordinates 4344624..4344899 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag OO857_RS21950 Protein ID WP_000593555.1
Coordinates 4344268..4344627 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO857_RS21920 (4339380) 4339380..4339853 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
OO857_RS21925 (4339850) 4339850..4340131 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
OO857_RS21930 (4340208) 4340208..4341566 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
OO857_RS21935 (4341559) 4341559..4343067 + 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
OO857_RS21940 (4343057) 4343057..4343326 + 270 WP_000933060.1 HPr family phosphocarrier protein -
OO857_RS21945 (4343358) 4343358..4344218 + 861 WP_001131788.1 class II aldolase -
OO857_RS21950 (4344268) 4344268..4344627 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
OO857_RS21955 (4344624) 4344624..4344899 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
OO857_RS21960 (4344972) 4344972..4346096 - 1125 WP_001301659.1 ABC transporter permease -
OO857_RS21965 (4346096) 4346096..4348831 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
OO857_RS21970 (4348828) 4348828..4349895 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T41823 WP_001259385.1 NZ_AP026080:c4344899-4344624 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T41823 NZ_AP026080:c4344899-4344624 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT41823 WP_000593555.1 NZ_AP026080:c4344627-4344268 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT41823 NZ_AP026080:c4344627-4344268 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References