Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3764700..3765354 Replicon chromosome
Accession NZ_AP026080
Organism Escherichia coli strain F690

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag OO857_RS18975 Protein ID WP_000244781.1
Coordinates 3764700..3765107 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OO857_RS18980 Protein ID WP_000354046.1
Coordinates 3765088..3765354 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO857_RS18955 (3760657) 3760657..3762390 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
OO857_RS18960 (3762396) 3762396..3763106 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OO857_RS18965 (3763131) 3763131..3764027 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OO857_RS18970 (3764139) 3764139..3764660 + 522 WP_001055874.1 flavodoxin FldB -
OO857_RS18975 (3764700) 3764700..3765107 - 408 WP_000244781.1 protein YgfX Toxin
OO857_RS18980 (3765088) 3765088..3765354 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OO857_RS18985 (3765597) 3765597..3766577 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
OO857_RS18990 (3766654) 3766654..3767313 - 660 WP_000250274.1 hemolysin III family protein -
OO857_RS18995 (3767477) 3767477..3767788 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
OO857_RS19000 (3767833) 3767833..3769266 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T41818 WP_000244781.1 NZ_AP026080:c3765107-3764700 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T41818 NZ_AP026080:c3765107-3764700 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT41818 WP_000354046.1 NZ_AP026080:c3765354-3765088 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT41818 NZ_AP026080:c3765354-3765088 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References