Detailed information of TA system TA041784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 332584..333166
• Replicon: chromosome
• Accession: NZ_AP025942
• Organism: Methylorubrum sp. GM97

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: C5B351
• Locus tag: MSPGM_RS01580
• Protein ID: WP_003600758.1
• Coordinates: 332891..333166 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: H1KK02
• Locus tag: MSPGM_RS01575
• Protein ID: WP_003600760.1
• Coordinates: 332584..332901 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSPGM_RS01560 (MSPGM_02980)	327856..328830	-	975	WP_003600763.1	aliphatic sulfonate ABC transporter substrate-binding protein	-
MSPGM_RS01565 (MSPGM_02990)	328990..331776	+	2787	WP_003600762.1	[protein-PII] uridylyltransferase	-
MSPGM_RS01570 (MSPGM_03000)	331868..332476	+	609	WP_003600761.1	nucleotide exchange factor GrpE	-
MSPGM_RS01575 (MSPGM_03010)	332584..332901	-	318	WP_003600760.1	BrnA antitoxin family protein	Antitoxin
MSPGM_RS01580 (MSPGM_03020)	332891..333166	-	276	WP_003600758.1	BrnT family toxin	Toxin
MSPGM_RS01585 (MSPGM_03030)	333258..334115	-	858	WP_003600757.1	prephenate dehydratase	-
MSPGM_RS01590 (MSPGM_03040)	334132..334875	-	744	WP_003600755.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
MSPGM_RS01595 (MSPGM_03050)	335183..336100	+	918	WP_003600753.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10095.62 Da
• Isoelectric Point: 8.6014

>T41784 WP_003600758.1 NZ_AP025942:c333166-332891 [Methylorubrum sp. GM97]
MLIVWDEPKRLTNLQKHGLDFADFEAGFDFETALVAVAQPSATGSARMKIIGEFDGQTVVAAIIAPLGREAISLISLRRA
SRSERRLYYAR

Nucleotide

• Length: 276 bp

>T41784 NZ_AP025942:c333166-332891 [Methylorubrum sp. GM97]
ATGTTGATTGTCTGGGACGAGCCCAAGCGCCTGACCAACCTGCAAAAGCACGGTCTCGACTTCGCGGATTTCGAGGCGGG
CTTCGACTTCGAGACCGCTCTTGTCGCGGTTGCCCAACCGAGCGCCACTGGCTCAGCCCGAATGAAGATCATCGGCGAGT
TCGACGGACAGACGGTGGTGGCAGCGATCATCGCTCCCTTGGGACGGGAAGCGATCTCGCTCATCAGCCTGCGCCGAGCC
AGCCGGAGCGAACGGAGGCTCTACTATGCCCGCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11641.04 Da
• Isoelectric Point: 5.1832

>AT41784 WP_003600760.1 NZ_AP025942:c332901-332584 [Methylorubrum sp. GM97]
MPADKPDRRPTTTSEEAGIQAGIAADPDNPEWTEADFRSAVPFEQAFPVLAEKLRRTRGPQSAPTKQLVSLRLDQDVLLR
FRATGPGWQSRMNEALRRAAADLPD

Nucleotide

• Length: 318 bp

>AT41784 NZ_AP025942:c332901-332584 [Methylorubrum sp. GM97]
ATGCCCGCTGACAAGCCCGATCGGAGACCGACCACGACCTCGGAGGAGGCCGGGATCCAAGCCGGCATCGCGGCCGATCC
CGACAATCCGGAATGGACCGAAGCGGACTTTCGAAGCGCCGTGCCCTTCGAACAGGCCTTTCCCGTCCTCGCAGAGAAAC
TGCGCCGCACCCGCGGCCCCCAAAGCGCTCCGACCAAACAGCTCGTCAGCTTGAGGCTCGATCAGGACGTCCTGCTTCGT
TTCAGGGCGACCGGTCCGGGCTGGCAGTCTCGGATGAACGAAGCCCTGCGCCGGGCCGCGGCCGATCTTCCCGACTGA

Structures

Toxin

• AlphaFold DB: C5B351

Antitoxin

• AlphaFold DB: H1KK02