Detailed information of TA system TA041748

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1082602..1083317
• Replicon: chromosome
• Accession: NZ_AP025931
• Organism: Enterobacter hormaechei strain FUJ80382

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: NGK50_RS05125
• Protein ID: WP_023303149.1
• Coordinates: 1082949..1083317 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: NGK50_RS05120
• Protein ID: WP_023303148.1
• Coordinates: 1082602..1082928 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK50_RS05095 (FJMB80382_09910)	1077636..1078901	+	1266	WP_023303143.1	hypothetical protein	-
NGK50_RS05100 (FJMB80382_09920)	1079210..1079644	+	435	WP_023303144.1	hypothetical protein	-
NGK50_RS05105 (FJMB80382_09930)	1079703..1080542	+	840	WP_023303145.1	hypothetical protein	-
NGK50_RS05110 (FJMB80382_09950)	1081232..1082053	+	822	WP_023303146.1	DUF932 domain-containing protein	-
NGK50_RS05115 (FJMB80382_09960)	1082119..1082589	+	471	WP_023303147.1	DNA repair protein RadC	-
NGK50_RS05120 (FJMB80382_09970)	1082602..1082928	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NGK50_RS05125 (FJMB80382_09980)	1082949..1083317	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
NGK50_RS05130 (FJMB80382_09990)	1083667..1084413	-	747	WP_032647364.1	class I SAM-dependent methyltransferase	-
NGK50_RS05135 (FJMB80382_10000)	1084424..1084681	-	258	WP_003863178.1	YjhX family toxin	-
NGK50_RS05140 (FJMB80382_10010)	1084941..1086878	-	1938	WP_032647366.1	bifunctional diguanylate cyclase/phosphodiesterase	-
NGK50_RS05145 (FJMB80382_10020)	1086896..1087390	-	495	WP_023295996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1061684..1083317	21633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T41748 WP_023303149.1 NZ_AP025931:1082949-1083317 [Enterobacter hormaechei]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T41748 NZ_AP025931:1082949-1083317 [Enterobacter hormaechei]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT41748 WP_023303148.1 NZ_AP025931:1082602-1082928 [Enterobacter hormaechei]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT41748 NZ_AP025931:1082602-1082928 [Enterobacter hormaechei]
ATGTCATTAATACCAGCCCATGAATGGGGGATAAAAAGCGATATTGTTCCACGGCTTGGAGTCAGATTAGTTCAGGAAGG
CAACCGGCTGCACTATCTGGCTGACCGGGCTAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCTGAAGTTGGATATCG
TATTTCCGCATTTTATCCGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATTCTCGCCATGCCCACTGTGTCACT
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCA
ACGCTAA