Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1221627..1222247 Replicon chromosome
Accession NZ_AP025927
Organism Enterobacter hormaechei strain FUJ80381

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGK75_RS05770 Protein ID WP_015571250.1
Coordinates 1221627..1221845 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGK75_RS05775 Protein ID WP_006809850.1
Coordinates 1221873..1222247 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK75_RS05740 (FJMB80381_11220) 1217639..1217899 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGK75_RS05745 1217902..1218042 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGK75_RS05750 (FJMB80381_11230) 1218039..1218749 - 711 WP_032647453.1 GNAT family protein -
NGK75_RS05755 (FJMB80381_11240) 1218851..1220311 + 1461 WP_032647455.1 PLP-dependent aminotransferase family protein -
NGK75_RS05760 (FJMB80381_11250) 1220283..1220750 - 468 WP_015571252.1 YlaC family protein -
NGK75_RS05765 (FJMB80381_11260) 1220867..1221418 - 552 WP_032647457.1 maltose O-acetyltransferase -
NGK75_RS05770 (FJMB80381_11270) 1221627..1221845 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGK75_RS05775 (FJMB80381_11280) 1221873..1222247 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGK75_RS05780 (FJMB80381_11290) 1222758..1225904 - 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
NGK75_RS05785 (FJMB80381_11300) 1225927..1227120 - 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T41730 WP_015571250.1 NZ_AP025927:c1221845-1221627 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T41730 NZ_AP025927:c1221845-1221627 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT41730 WP_006809850.1 NZ_AP025927:c1222247-1221873 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT41730 NZ_AP025927:c1222247-1221873 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References