Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1868928..1869725 Replicon chromosome
Accession NZ_AP025895
Organism Enterobacter hormaechei strain FUJ80191

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag NGK38_RS08890 Protein ID WP_032647926.1
Coordinates 1869204..1869725 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag NGK38_RS08885 Protein ID WP_015570876.1
Coordinates 1868928..1869197 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK38_RS08850 (FJMB80191_17330) 1864468..1864827 + 360 WP_032647921.1 helix-turn-helix domain-containing protein -
NGK38_RS08855 1864906..1865001 - 96 Protein_1722 transcriptional regulator -
NGK38_RS08860 (FJMB80191_17350) 1865154..1866395 + 1242 WP_032647923.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
NGK38_RS08865 (FJMB80191_17360) 1866428..1866655 - 228 WP_006809324.1 YccJ family protein -
NGK38_RS08870 (FJMB80191_17370) 1866674..1867270 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
NGK38_RS08875 (FJMB80191_17380) 1867662..1867832 + 171 WP_001273664.1 general stress protein -
NGK38_RS08880 (FJMB80191_17390) 1867949..1868854 + 906 WP_017693666.1 DMT family transporter -
NGK38_RS08885 (FJMB80191_17400) 1868928..1869197 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
NGK38_RS08890 (FJMB80191_17410) 1869204..1869725 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
NGK38_RS08895 (FJMB80191_17420) 1869784..1871106 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
NGK38_RS08900 (FJMB80191_17430) 1871128..1871619 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
NGK38_RS08905 (FJMB80191_17440) 1871632..1872222 - 591 WP_032647932.1 malonic semialdehyde reductase -
NGK38_RS08910 (FJMB80191_17450) 1872232..1873032 - 801 WP_032647935.1 pyrimidine utilization protein D -
NGK38_RS08915 (FJMB80191_17460) 1873040..1873426 - 387 WP_015570870.1 pyrimidine utilization protein C -
NGK38_RS08920 (FJMB80191_17470) 1873438..1874127 - 690 WP_017384771.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T41579 WP_032647926.1 NZ_AP025895:1869204-1869725 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T41579 NZ_AP025895:1869204-1869725 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT41579 WP_015570876.1 NZ_AP025895:1868928-1869197 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT41579 NZ_AP025895:1868928-1869197 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References