Detailed information of TA system TA041576

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1085007..1085722
• Replicon: chromosome
• Accession: NZ_AP025895
• Organism: Enterobacter hormaechei strain FUJ80191

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: NGK38_RS05145
• Protein ID: WP_023303149.1
• Coordinates: 1085354..1085722 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: NGK38_RS05140
• Protein ID: WP_023303148.1
• Coordinates: 1085007..1085333 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK38_RS05115 (FJMB80191_09930)	1080041..1081306	+	1266	WP_023303143.1	hypothetical protein	-
NGK38_RS05120 (FJMB80191_09940)	1081615..1082049	+	435	WP_023303144.1	hypothetical protein	-
NGK38_RS05125 (FJMB80191_09950)	1082108..1082947	+	840	WP_023303145.1	hypothetical protein	-
NGK38_RS05130 (FJMB80191_09970)	1083637..1084458	+	822	WP_023303146.1	DUF932 domain-containing protein	-
NGK38_RS05135 (FJMB80191_09980)	1084524..1084994	+	471	WP_023303147.1	DNA repair protein RadC	-
NGK38_RS05140 (FJMB80191_09990)	1085007..1085333	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NGK38_RS05145 (FJMB80191_10000)	1085354..1085722	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
NGK38_RS05150 (FJMB80191_10010)	1086072..1086818	-	747	WP_032647364.1	class I SAM-dependent methyltransferase	-
NGK38_RS05155 (FJMB80191_10020)	1086829..1087086	-	258	WP_003863178.1	YjhX family toxin	-
NGK38_RS05160 (FJMB80191_10030)	1087346..1089283	-	1938	WP_032647366.1	bifunctional diguanylate cyclase/phosphodiesterase	-
NGK38_RS05165 (FJMB80191_10040)	1089301..1089795	-	495	WP_023295996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1064089..1085722	21633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T41576 WP_023303149.1 NZ_AP025895:1085354-1085722 [Enterobacter hormaechei]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T41576 NZ_AP025895:1085354-1085722 [Enterobacter hormaechei]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT41576 WP_023303148.1 NZ_AP025895:1085007-1085333 [Enterobacter hormaechei]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT41576 NZ_AP025895:1085007-1085333 [Enterobacter hormaechei]
ATGTCATTAATACCAGCCCATGAATGGGGGATAAAAAGCGATATTGTTCCACGGCTTGGAGTCAGATTAGTTCAGGAAGG
CAACCGGCTGCACTATCTGGCTGACCGGGCTAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCTGAAGTTGGATATCG
TATTTCCGCATTTTATCCGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATTCTCGCCATGCCCACTGTGTCACT
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCA
ACGCTAA