Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 728161..728737 Replicon chromosome
Accession NZ_AP025891
Organism Enterobacter hormaechei strain FUJ80182

Toxin (Protein)


Gene name shpA Uniprot ID A0A800YKM1
Locus tag NGJ49_RS03470 Protein ID WP_015572580.1
Coordinates 728161..728448 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A801DSF4
Locus tag NGJ49_RS03475 Protein ID WP_017694570.1
Coordinates 728435..728737 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGJ49_RS03445 (FJMB80182_06770) 723279..723974 + 696 WP_032647138.1 winged helix-turn-helix domain-containing protein -
NGJ49_RS03450 (FJMB80182_06780) 724124..724594 + 471 WP_032647140.1 MarR family transcriptional regulator -
NGJ49_RS03455 (FJMB80182_06790) 724591..725658 + 1068 WP_026094404.1 HlyD family secretion protein -
NGJ49_RS03460 (FJMB80182_06800) 725648..726724 + 1077 WP_015572578.1 DUF2955 domain-containing protein -
NGJ49_RS03465 (FJMB80182_06810) 726721..727992 - 1272 WP_032647142.1 DUF445 domain-containing protein -
NGJ49_RS03470 (FJMB80182_06820) 728161..728448 + 288 WP_015572580.1 BrnT family toxin Toxin
NGJ49_RS03475 (FJMB80182_06830) 728435..728737 + 303 WP_017694570.1 BrnA antitoxin family protein Antitoxin
NGJ49_RS03480 (FJMB80182_06840) 728766..729404 - 639 WP_032647144.1 LysE family translocator -
NGJ49_RS03485 (FJMB80182_06850) 729443..730195 - 753 WP_015572582.1 AraC family transcriptional regulator -
NGJ49_RS03490 (FJMB80182_06860) 730349..731719 + 1371 WP_032647147.1 NAD-dependent succinate-semialdehyde dehydrogenase -
NGJ49_RS03495 (FJMB80182_06870) 731897..732442 - 546 WP_006810254.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11296.81 Da        Isoelectric Point: 7.3587

>T41556 WP_015572580.1 NZ_AP025891:728161-728448 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Download         Length: 288 bp

>T41556 NZ_AP025891:728161-728448 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGTGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11482.13 Da        Isoelectric Point: 10.4094

>AT41556 WP_017694570.1 NZ_AP025891:728435-728737 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT41556 NZ_AP025891:728435-728737 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References