Detailed information of TA system TA041540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1756617..1757530
• Replicon: chromosome
• Accession: NZ_AP025887
• Organism: Enterobacter hormaechei strain FUJ80178

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: NGK69_RS08390
• Protein ID: WP_032647883.1
• Coordinates: 1756617..1757090 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: NGK69_RS08395
• Protein ID: WP_023296216.1
• Coordinates: 1757087..1757530 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK69_RS08370 (FJMB80178_16410)	1752776..1754086	+	1311	WP_032647879.1	type I-F CRISPR-associated protein Csy1	-
NGK69_RS08375 (FJMB80178_16420)	1754083..1755018	+	936	WP_032647880.1	type I-F CRISPR-associated protein Csy2	-
NGK69_RS08380 (FJMB80178_16430)	1755032..1756030	+	999	WP_032647881.1	type I-F CRISPR-associated protein Csy3	-
NGK69_RS08385 (FJMB80178_16440)	1756040..1756594	+	555	WP_032647882.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
NGK69_RS08390 (FJMB80178_16450)	1756617..1757090	-	474	WP_032647883.1	RES domain-containing protein	Toxin
NGK69_RS08395 (FJMB80178_16460)	1757087..1757530	-	444	WP_023296216.1	DUF2384 domain-containing protein	Antitoxin
NGK69_RS08400 (FJMB80178_16470)	1758628..1758846	-	219	WP_002211347.1	translation initiation factor IF-1	-
NGK69_RS08405 (FJMB80178_16480)	1759131..1759835	-	705	WP_032668747.1	leucyl/phenylalanyl-tRNA--protein transferase	-
NGK69_RS08410 (FJMB80178_16490)	1759881..1761602	-	1722	WP_032647885.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-147)

Antitoxin

• Antitoxin Xre (15-147)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17569.96 Da
• Isoelectric Point: 5.5555

>T41540 WP_032647883.1 NZ_AP025887:c1757090-1756617 [Enterobacter hormaechei]
MIFYRLVTGRFASEAWSGSGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVRKDTVLNQYQLFSINIPDDQIDYLDKA
WLPEDWQENPAPVSTMDLGTGWLQANSALALILPSCIIPYENNAILNPLHPAFRKALNSVELLPFNFDTRLAEKTAP

Nucleotide

• Length: 474 bp

>T41540 NZ_AP025887:c1757090-1756617 [Enterobacter hormaechei]
ATGATTTTTTATCGTCTGGTGACAGGACGTTTTGCCAGCGAAGCGTGGAGTGGCAGTGGTGCAAACCAGTATGGCGGACG
CTGGAATCATAAAGGCCATCCTGCCGTGTATGTCTCCACCTCCATCTCGCTGGCTTCACTGGAAATTCTGGTGCATGTCA
GAAAAGACACCGTGCTGAACCAGTACCAGCTTTTCAGCATTAATATCCCGGATGATCAGATTGATTACCTGGATAAAGCG
TGGCTGCCCGAGGACTGGCAGGAAAATCCGGCGCCGGTATCAACGATGGATCTGGGTACCGGTTGGTTGCAGGCTAACAG
CGCTCTCGCACTCATCTTACCCTCCTGCATCATTCCCTATGAAAACAACGCCATACTTAATCCATTGCATCCGGCATTCC
GCAAAGCACTTAATTCTGTTGAGTTACTGCCTTTTAATTTTGATACCCGTCTCGCAGAAAAAACCGCACCCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16512.81 Da
• Isoelectric Point: 6.4819

>AT41540 WP_023296216.1 NZ_AP025887:c1757530-1757087 [Enterobacter hormaechei]
MRTWVPAQRPADNTLWRYAGLPASRGMRLIEYLNQGLPVSVLDNIHEWTDMSKAEILRVTGINERNVARRKSTGRTLTPD
ESERVARFVRVLDAAVDYFGSKEEAWNWLQLPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT41540 NZ_AP025887:c1757530-1757087 [Enterobacter hormaechei]
ATGAGAACATGGGTTCCTGCGCAAAGGCCGGCTGATAACACGCTGTGGCGCTACGCCGGTTTACCTGCAAGCAGAGGCAT
GCGGCTGATTGAATATCTCAATCAAGGATTACCCGTCAGCGTCCTCGACAATATTCACGAGTGGACGGACATGTCGAAAG
CGGAGATTTTACGCGTCACGGGAATTAACGAGCGTAACGTTGCCCGCCGCAAAAGTACGGGCCGGACGCTGACGCCCGAC
GAGAGCGAACGTGTGGCGCGTTTCGTTCGGGTACTGGATGCGGCCGTCGATTATTTCGGCAGTAAAGAAGAGGCATGGAA
CTGGTTGCAGTTACCGGTACGTGGGCTTGGCAATGTTGCGCCTGTCGATCTGATTGCCACGGAAACCGGTGCGCTGGAAG
TTACCGATTTGATTGGTCGGCTGGAGCACGGAGTGTTCGCATGA