Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1867865..1868662 Replicon chromosome
Accession NZ_AP025867
Organism Enterobacter hormaechei strain FUJ80151

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag NGK26_RS08880 Protein ID WP_032647926.1
Coordinates 1868141..1868662 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag NGK26_RS08875 Protein ID WP_015570876.1
Coordinates 1867865..1868134 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK26_RS08840 (FJMB80151_17290) 1863405..1863764 + 360 WP_032647921.1 helix-turn-helix domain-containing protein -
NGK26_RS08845 1863843..1863938 - 96 Protein_1720 transcriptional regulator -
NGK26_RS08850 (FJMB80151_17310) 1864091..1865332 + 1242 WP_032647923.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
NGK26_RS08855 (FJMB80151_17320) 1865365..1865592 - 228 WP_006809324.1 YccJ family protein -
NGK26_RS08860 (FJMB80151_17330) 1865611..1866207 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
NGK26_RS08865 (FJMB80151_17340) 1866599..1866769 + 171 WP_001273664.1 general stress protein -
NGK26_RS08870 (FJMB80151_17350) 1866886..1867791 + 906 WP_017693666.1 DMT family transporter -
NGK26_RS08875 (FJMB80151_17360) 1867865..1868134 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
NGK26_RS08880 (FJMB80151_17370) 1868141..1868662 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
NGK26_RS08885 (FJMB80151_17380) 1868721..1870043 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
NGK26_RS08890 (FJMB80151_17390) 1870065..1870556 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
NGK26_RS08895 (FJMB80151_17400) 1870569..1871159 - 591 WP_032647932.1 malonic semialdehyde reductase -
NGK26_RS08900 (FJMB80151_17410) 1871169..1871969 - 801 WP_032647935.1 pyrimidine utilization protein D -
NGK26_RS08905 (FJMB80151_17420) 1871977..1872363 - 387 WP_015570870.1 pyrimidine utilization protein C -
NGK26_RS08910 (FJMB80151_17430) 1872375..1873064 - 690 WP_017384771.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T41447 WP_032647926.1 NZ_AP025867:1868141-1868662 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T41447 NZ_AP025867:1868141-1868662 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT41447 WP_015570876.1 NZ_AP025867:1867865-1868134 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT41447 NZ_AP025867:1867865-1868134 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References