Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1222967..1223587 Replicon chromosome
Accession NZ_AP025867
Organism Enterobacter hormaechei strain FUJ80151

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGK26_RS05780 Protein ID WP_015571250.1
Coordinates 1222967..1223185 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGK26_RS05785 Protein ID WP_006809850.1
Coordinates 1223213..1223587 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK26_RS05750 (FJMB80151_11210) 1218979..1219239 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGK26_RS05755 1219242..1219382 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGK26_RS05760 (FJMB80151_11220) 1219379..1220089 - 711 WP_032647453.1 GNAT family protein -
NGK26_RS05765 (FJMB80151_11230) 1220191..1221651 + 1461 WP_032647455.1 PLP-dependent aminotransferase family protein -
NGK26_RS05770 (FJMB80151_11240) 1221623..1222090 - 468 WP_015571252.1 YlaC family protein -
NGK26_RS05775 (FJMB80151_11250) 1222207..1222758 - 552 WP_032647457.1 maltose O-acetyltransferase -
NGK26_RS05780 (FJMB80151_11260) 1222967..1223185 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGK26_RS05785 (FJMB80151_11270) 1223213..1223587 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGK26_RS05790 (FJMB80151_11280) 1224098..1227244 - 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
NGK26_RS05795 (FJMB80151_11290) 1227267..1228460 - 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T41445 WP_015571250.1 NZ_AP025867:c1223185-1222967 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T41445 NZ_AP025867:c1223185-1222967 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT41445 WP_006809850.1 NZ_AP025867:c1223587-1223213 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT41445 NZ_AP025867:c1223587-1223213 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References