Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1866526..1867323 Replicon chromosome
Accession NZ_AP025863
Organism Enterobacter hormaechei strain FUJ80146

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag NGJ48_RS08870 Protein ID WP_032647926.1
Coordinates 1866802..1867323 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag NGJ48_RS08865 Protein ID WP_015570876.1
Coordinates 1866526..1866795 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGJ48_RS08830 (FJMB80146_17310) 1862066..1862425 + 360 WP_032647921.1 helix-turn-helix domain-containing protein -
NGJ48_RS08835 1862504..1862599 - 96 Protein_1718 transcriptional regulator -
NGJ48_RS08840 (FJMB80146_17330) 1862752..1863993 + 1242 WP_032647923.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
NGJ48_RS08845 (FJMB80146_17340) 1864026..1864253 - 228 WP_006809324.1 YccJ family protein -
NGJ48_RS08850 (FJMB80146_17350) 1864272..1864868 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
NGJ48_RS08855 (FJMB80146_17360) 1865260..1865430 + 171 WP_001273664.1 general stress protein -
NGJ48_RS08860 (FJMB80146_17370) 1865547..1866452 + 906 WP_017693666.1 DMT family transporter -
NGJ48_RS08865 (FJMB80146_17380) 1866526..1866795 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
NGJ48_RS08870 (FJMB80146_17390) 1866802..1867323 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
NGJ48_RS08875 (FJMB80146_17400) 1867382..1868704 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
NGJ48_RS08880 (FJMB80146_17410) 1868726..1869217 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
NGJ48_RS08885 (FJMB80146_17420) 1869230..1869820 - 591 WP_032647932.1 malonic semialdehyde reductase -
NGJ48_RS08890 (FJMB80146_17430) 1869830..1870630 - 801 WP_032647935.1 pyrimidine utilization protein D -
NGJ48_RS08895 (FJMB80146_17440) 1870638..1871024 - 387 WP_015570870.1 pyrimidine utilization protein C -
NGJ48_RS08900 (FJMB80146_17450) 1871036..1871725 - 690 WP_017384771.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T41428 WP_032647926.1 NZ_AP025863:1866802-1867323 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T41428 NZ_AP025863:1866802-1867323 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT41428 WP_015570876.1 NZ_AP025863:1866526-1866795 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT41428 NZ_AP025863:1866526-1866795 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References