Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1866527..1867324 Replicon chromosome
Accession NZ_AP025855
Organism Enterobacter hormaechei strain FUJ80144

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag NGJ60_RS08870 Protein ID WP_032647926.1
Coordinates 1866803..1867324 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag NGJ60_RS08865 Protein ID WP_015570876.1
Coordinates 1866527..1866796 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGJ60_RS08830 (FJMB80144_17290) 1862067..1862426 + 360 WP_032647921.1 helix-turn-helix domain-containing protein -
NGJ60_RS08835 1862505..1862600 - 96 Protein_1718 transcriptional regulator -
NGJ60_RS08840 (FJMB80144_17310) 1862753..1863994 + 1242 WP_032647923.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
NGJ60_RS08845 (FJMB80144_17320) 1864027..1864254 - 228 WP_006809324.1 YccJ family protein -
NGJ60_RS08850 (FJMB80144_17330) 1864273..1864869 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
NGJ60_RS08855 (FJMB80144_17340) 1865261..1865431 + 171 WP_001273664.1 general stress protein -
NGJ60_RS08860 (FJMB80144_17350) 1865548..1866453 + 906 WP_017693666.1 DMT family transporter -
NGJ60_RS08865 (FJMB80144_17360) 1866527..1866796 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
NGJ60_RS08870 (FJMB80144_17370) 1866803..1867324 + 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
NGJ60_RS08875 (FJMB80144_17380) 1867383..1868705 - 1323 WP_032647928.1 pyrimidine utilization transport protein G -
NGJ60_RS08880 (FJMB80144_17390) 1868727..1869218 - 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
NGJ60_RS08885 (FJMB80144_17400) 1869231..1869821 - 591 WP_032647932.1 malonic semialdehyde reductase -
NGJ60_RS08890 (FJMB80144_17410) 1869831..1870631 - 801 WP_032647935.1 pyrimidine utilization protein D -
NGJ60_RS08895 (FJMB80144_17420) 1870639..1871025 - 387 WP_015570870.1 pyrimidine utilization protein C -
NGJ60_RS08900 (FJMB80144_17430) 1871037..1871726 - 690 WP_017384771.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T41390 WP_032647926.1 NZ_AP025855:1866803-1867324 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T41390 NZ_AP025855:1866803-1867324 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT41390 WP_015570876.1 NZ_AP025855:1866527-1866796 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT41390 NZ_AP025855:1866527-1866796 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References