Detailed information of TA system TA041368

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1091610..1092325
• Replicon: chromosome
• Accession: NZ_AP025851
• Organism: Enterobacter hormaechei strain FUJ80068

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: NGJ26_RS05175
• Protein ID: WP_023303149.1
• Coordinates: 1091957..1092325 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: NGJ26_RS05170
• Protein ID: WP_023303148.1
• Coordinates: 1091610..1091936 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ26_RS05145 (FJMB80068_10050)	1086644..1087909	+	1266	WP_023303143.1	hypothetical protein	-
NGJ26_RS05150 (FJMB80068_10060)	1088218..1088652	+	435	WP_023303144.1	hypothetical protein	-
NGJ26_RS05155 (FJMB80068_10070)	1088711..1089550	+	840	WP_023303145.1	hypothetical protein	-
NGJ26_RS05160 (FJMB80068_10090)	1090240..1091061	+	822	WP_023303146.1	DUF932 domain-containing protein	-
NGJ26_RS05165 (FJMB80068_10100)	1091127..1091597	+	471	WP_023303147.1	DNA repair protein RadC	-
NGJ26_RS05170 (FJMB80068_10110)	1091610..1091936	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NGJ26_RS05175 (FJMB80068_10120)	1091957..1092325	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
NGJ26_RS05180 (FJMB80068_10130)	1092675..1093421	-	747	WP_032647364.1	class I SAM-dependent methyltransferase	-
NGJ26_RS05185 (FJMB80068_10140)	1093432..1093689	-	258	WP_003863178.1	YjhX family toxin	-
NGJ26_RS05190 (FJMB80068_10150)	1093949..1095886	-	1938	WP_032647366.1	bifunctional diguanylate cyclase/phosphodiesterase	-
NGJ26_RS05195 (FJMB80068_10160)	1095904..1096398	-	495	WP_023295996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T41368 WP_023303149.1 NZ_AP025851:1091957-1092325 [Enterobacter hormaechei]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T41368 NZ_AP025851:1091957-1092325 [Enterobacter hormaechei]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT41368 WP_023303148.1 NZ_AP025851:1091610-1091936 [Enterobacter hormaechei]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT41368 NZ_AP025851:1091610-1091936 [Enterobacter hormaechei]
ATGTCATTAATACCAGCCCATGAATGGGGGATAAAAAGCGATATTGTTCCACGGCTTGGAGTCAGATTAGTTCAGGAAGG
CAACCGGCTGCACTATCTGGCTGACCGGGCTAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCTGAAGTTGGATATCG
TATTTCCGCATTTTATCCGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATTCTCGCCATGCCCACTGTGTCACT
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCA
ACGCTAA