Detailed information of TA system TA041349

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1091611..1092326
• Replicon: chromosome
• Accession: NZ_AP025847
• Organism: Enterobacter hormaechei strain FUJ80063

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: NGK57_RS05175
• Protein ID: WP_023303149.1
• Coordinates: 1091958..1092326 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: NGK57_RS05170
• Protein ID: WP_023303148.1
• Coordinates: 1091611..1091937 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK57_RS05145 (FJMB80063_10050)	1086645..1087910	+	1266	WP_023303143.1	hypothetical protein	-
NGK57_RS05150 (FJMB80063_10060)	1088219..1088653	+	435	WP_023303144.1	hypothetical protein	-
NGK57_RS05155 (FJMB80063_10070)	1088712..1089551	+	840	WP_023303145.1	hypothetical protein	-
NGK57_RS05160 (FJMB80063_10090)	1090241..1091062	+	822	WP_023303146.1	DUF932 domain-containing protein	-
NGK57_RS05165 (FJMB80063_10100)	1091128..1091598	+	471	WP_023303147.1	DNA repair protein RadC	-
NGK57_RS05170 (FJMB80063_10110)	1091611..1091937	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NGK57_RS05175 (FJMB80063_10120)	1091958..1092326	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
NGK57_RS05180 (FJMB80063_10130)	1092676..1093422	-	747	WP_032647364.1	class I SAM-dependent methyltransferase	-
NGK57_RS05185 (FJMB80063_10140)	1093433..1093690	-	258	WP_003863178.1	YjhX family toxin	-
NGK57_RS05190 (FJMB80063_10150)	1093950..1095887	-	1938	WP_032647366.1	bifunctional diguanylate cyclase/phosphodiesterase	-
NGK57_RS05195 (FJMB80063_10160)	1095905..1096399	-	495	WP_023295996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T41349 WP_023303149.1 NZ_AP025847:1091958-1092326 [Enterobacter hormaechei]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T41349 NZ_AP025847:1091958-1092326 [Enterobacter hormaechei]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT41349 WP_023303148.1 NZ_AP025847:1091611-1091937 [Enterobacter hormaechei]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT41349 NZ_AP025847:1091611-1091937 [Enterobacter hormaechei]
ATGTCATTAATACCAGCCCATGAATGGGGGATAAAAAGCGATATTGTTCCACGGCTTGGAGTCAGATTAGTTCAGGAAGG
CAACCGGCTGCACTATCTGGCTGACCGGGCTAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCTGAAGTTGGATATCG
TATTTCCGCATTTTATCCGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATTCTCGCCATGCCCACTGTGTCACT
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCA
ACGCTAA