Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3612409..3613030 Replicon chromosome
Accession NZ_AP025842
Organism Enterobacter hormaechei strain FUJ80379

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NMC33_RS17495 Protein ID WP_015571250.1
Coordinates 3612812..3613030 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NMC33_RS17490 Protein ID WP_006809850.1
Coordinates 3612409..3612783 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMC33_RS17480 (FJMB80379_34270) 3607537..3608730 + 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
NMC33_RS17485 (FJMB80379_34280) 3608753..3611899 + 3147 WP_126493935.1 multidrug efflux RND transporter permease subunit AcrB -
NMC33_RS17490 (FJMB80379_34290) 3612409..3612783 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NMC33_RS17495 (FJMB80379_34300) 3612812..3613030 + 219 WP_015571250.1 HHA domain-containing protein Toxin
NMC33_RS17500 (FJMB80379_34310) 3613238..3613789 + 552 WP_022647270.1 maltose O-acetyltransferase -
NMC33_RS17505 (FJMB80379_34320) 3613906..3614373 + 468 WP_022647269.1 YlaC family protein -
NMC33_RS17510 (FJMB80379_34330) 3614345..3615805 - 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
NMC33_RS17515 (FJMB80379_34340) 3615908..3616618 + 711 WP_022647267.1 GNAT family protein -
NMC33_RS17520 3616615..3616755 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NMC33_RS17525 (FJMB80379_34350) 3616758..3617018 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T41344 WP_015571250.1 NZ_AP025842:3612812-3613030 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T41344 NZ_AP025842:3612812-3613030 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT41344 WP_006809850.1 NZ_AP025842:3612409-3612783 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT41344 NZ_AP025842:3612409-3612783 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References