Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2276959..2277698 Replicon chromosome
Accession NZ_AP025839
Organism Enterobacter hormaechei strain FUJ80299

Toxin (Protein)


Gene name tacT Uniprot ID A0A3S0FZ61
Locus tag NGK29_RS11040 Protein ID WP_022648141.1
Coordinates 2276959..2277444 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag NGK29_RS11045 Protein ID WP_003857131.1
Coordinates 2277432..2277698 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK29_RS11010 2272485..2273693 + 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
NGK29_RS11015 (FJMB80299_21620) 2273706..2274137 + 432 WP_251259014.1 alpha/beta hydrolase -
NGK29_RS11020 (FJMB80299_21630) 2274233..2274997 + 765 WP_022648136.1 SDR family oxidoreductase -
NGK29_RS11025 (FJMB80299_21650) 2275664..2275927 + 264 WP_022648138.1 DUF4225 domain-containing protein -
NGK29_RS11030 (FJMB80299_21660) 2275902..2276231 - 330 WP_022648139.1 hypothetical protein -
NGK29_RS11035 (FJMB80299_21670) 2276364..2276933 + 570 WP_022648140.1 hypothetical protein -
NGK29_RS11040 (FJMB80299_21680) 2276959..2277444 - 486 WP_022648141.1 GNAT family N-acetyltransferase Toxin
NGK29_RS11045 (FJMB80299_21690) 2277432..2277698 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
NGK29_RS11050 (FJMB80299_21700) 2277762..2278691 - 930 WP_022648142.1 LysR family transcriptional regulator -
NGK29_RS11055 (FJMB80299_21710) 2278821..2280209 + 1389 WP_022648143.1 MFS transporter -
NGK29_RS11060 (FJMB80299_21720) 2280188..2280745 - 558 WP_022648144.1 OmpH family outer membrane protein -
NGK29_RS11065 (FJMB80299_21730) 2280866..2282002 - 1137 WP_022648145.1 type 1 fimbrial protein -
NGK29_RS11070 (FJMB80299_21740) 2282026..2282604 - 579 WP_022648146.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2252754..2277698 24944
- flank IS/Tn - - 2272485..2273693 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17558.24 Da        Isoelectric Point: 9.6275

>T41320 WP_022648141.1 NZ_AP025839:c2277444-2276959 [Enterobacter hormaechei]
VGRVTPPEPLSSVHQLAEFISGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T41320 NZ_AP025839:c2277444-2276959 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT41320 WP_003857131.1 NZ_AP025839:c2277698-2277432 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT41320 NZ_AP025839:c2277698-2277432 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S0FZ61

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References