Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 117158..118781 Replicon plasmid pFJMB80169_1
Accession NZ_AP025831
Organism Enterobacter hormaechei strain FUJ80169

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag NGK35_RS22910 Protein ID WP_000088044.1
Coordinates 117456..118781 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag NGK35_RS22905 Protein ID WP_012477377.1
Coordinates 117158..117451 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK35_RS22885 (FJMB80169_44760) 112957..113349 + 393 WP_000341066.1 hypothetical protein -
NGK35_RS22890 (FJMB80169_44770) 114008..114664 + 657 WP_000462754.1 trypsin-like serine protease -
NGK35_RS22895 (FJMB80169_44790) 114867..115364 + 498 WP_087878322.1 hypothetical protein -
NGK35_RS22900 (FJMB80169_44800) 115369..116757 + 1389 WP_000083579.1 hypothetical protein -
NGK35_RS22905 (FJMB80169_44810) 117158..117451 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
NGK35_RS22910 (FJMB80169_44820) 117456..118781 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
NGK35_RS22915 (FJMB80169_44830) 118842..119048 + 207 WP_000134171.1 hypothetical protein -
NGK35_RS22920 (FJMB80169_44840) 119149..119559 + 411 WP_000985909.1 hypothetical protein -
NGK35_RS22925 (FJMB80169_44850) 119572..120387 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
NGK35_RS22930 (FJMB80169_44860) 120640..121065 + 426 WP_000278471.1 hypothetical protein -
NGK35_RS22935 (FJMB80169_44870) 121614..121922 + 309 WP_001224686.1 hypothetical protein -
NGK35_RS22940 (FJMB80169_44880) 121938..122795 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aac(6')-IIc / blaIMP-1 / qnrB6 / tet(B) - 1..371458 371458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T41285 WP_000088044.1 NZ_AP025831:117456-118781 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T41285 NZ_AP025831:117456-118781 [Enterobacter hormaechei]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT41285 WP_012477377.1 NZ_AP025831:117158-117451 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT41285 NZ_AP025831:117158-117451 [Enterobacter hormaechei]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References