Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3799512..3800293 Replicon chromosome
Accession NZ_AP025830
Organism Enterobacter hormaechei strain FUJ80169

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A431SHU0
Locus tag NGK35_RS18060 Protein ID WP_022649378.1
Coordinates 3799512..3800003 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A3S0FY21
Locus tag NGK35_RS18065 Protein ID WP_022649379.1
Coordinates 3800000..3800293 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK35_RS18045 (FJMB80169_35280) 3794792..3795244 - 453 WP_022649375.1 hypothetical protein -
NGK35_RS18050 (FJMB80169_35290) 3795759..3797669 + 1911 WP_022649376.1 hypothetical protein -
NGK35_RS18055 (FJMB80169_35300) 3797666..3799360 + 1695 WP_022649377.1 hypothetical protein -
NGK35_RS18060 (FJMB80169_35310) 3799512..3800003 - 492 WP_022649378.1 GNAT family N-acetyltransferase Toxin
NGK35_RS18065 (FJMB80169_35320) 3800000..3800293 - 294 WP_022649379.1 DUF1778 domain-containing protein Antitoxin
NGK35_RS18070 (FJMB80169_35330) 3800395..3802188 - 1794 WP_022649380.1 hybrid sensor histidine kinase/response regulator -
NGK35_RS18075 (FJMB80169_35340) 3802185..3802817 - 633 WP_022649381.1 response regulator transcription factor -
NGK35_RS18080 (FJMB80169_35350) 3802993..3803307 + 315 WP_003862457.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3794792..3803307 8515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(83-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17469.36 Da        Isoelectric Point: 8.1405

>T41283 WP_022649378.1 NZ_AP025830:c3800003-3799512 [Enterobacter hormaechei]
MISAPVPLRAGHILTPFCCGVDSMDNWLKQRAMKNQASGASRTFVCCGGDSNVLAYYALASSAVTTNTVPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAESIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLV
ASI

Download         Length: 492 bp

>T41283 NZ_AP025830:c3800003-3799512 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10959.58 Da        Isoelectric Point: 8.6351

>AT41283 WP_022649379.1 NZ_AP025830:c3800293-3800000 [Enterobacter hormaechei]
MPAANTMAVKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLTRLDQAPAPN
AALRKTMQTLAPWEQEK

Download         Length: 294 bp

>AT41283 NZ_AP025830:c3800293-3800000 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A431SHU0

Antitoxin

Source ID Structure
AlphaFold DB A0A3S0FY21

References