Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2201310..2202049 Replicon chromosome
Accession NZ_AP025827
Organism Enterobacter hormaechei strain FUJ80149

Toxin (Protein)


Gene name tacT Uniprot ID A0A3S0FZ61
Locus tag NGK41_RS10475 Protein ID WP_022648141.1
Coordinates 2201310..2201795 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag NGK41_RS10480 Protein ID WP_003857131.1
Coordinates 2201783..2202049 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK41_RS10445 2196836..2198044 + 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
NGK41_RS10450 (FJMB80149_20430) 2198057..2198488 + 432 WP_251259014.1 alpha/beta hydrolase -
NGK41_RS10455 (FJMB80149_20440) 2198584..2199348 + 765 WP_022648136.1 SDR family oxidoreductase -
NGK41_RS10460 (FJMB80149_20460) 2200015..2200278 + 264 WP_022648138.1 DUF4225 domain-containing protein -
NGK41_RS10465 (FJMB80149_20470) 2200253..2200582 - 330 WP_022648139.1 hypothetical protein -
NGK41_RS10470 (FJMB80149_20480) 2200715..2201284 + 570 WP_022648140.1 hypothetical protein -
NGK41_RS10475 (FJMB80149_20490) 2201310..2201795 - 486 WP_022648141.1 GNAT family N-acetyltransferase Toxin
NGK41_RS10480 (FJMB80149_20500) 2201783..2202049 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
NGK41_RS10485 (FJMB80149_20510) 2202113..2203042 - 930 WP_022648142.1 LysR family transcriptional regulator -
NGK41_RS10490 (FJMB80149_20520) 2203172..2204560 + 1389 WP_022648143.1 MFS transporter -
NGK41_RS10495 (FJMB80149_20530) 2204539..2205096 - 558 WP_022648144.1 OmpH family outer membrane protein -
NGK41_RS10500 (FJMB80149_20540) 2205217..2206353 - 1137 WP_022648145.1 type 1 fimbrial protein -
NGK41_RS10505 (FJMB80149_20550) 2206377..2206955 - 579 WP_022648146.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2177105..2205096 27991
- flank IS/Tn - - 2196836..2198044 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17558.24 Da        Isoelectric Point: 9.6275

>T41256 WP_022648141.1 NZ_AP025827:c2201795-2201310 [Enterobacter hormaechei]
VGRVTPPEPLSSVHQLAEFISGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T41256 NZ_AP025827:c2201795-2201310 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT41256 WP_003857131.1 NZ_AP025827:c2202049-2201783 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT41256 NZ_AP025827:c2202049-2201783 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S0FZ61

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References