Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3843089..3843870 Replicon chromosome
Accession NZ_AP025812
Organism Enterobacter hormaechei strain FUJ80062

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A431SHU0
Locus tag NGK32_RS18350 Protein ID WP_022649378.1
Coordinates 3843089..3843580 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A3S0FY21
Locus tag NGK32_RS18355 Protein ID WP_022649379.1
Coordinates 3843577..3843870 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK32_RS18335 (FJMB80062_35720) 3838369..3838821 - 453 WP_022649375.1 hypothetical protein -
NGK32_RS18340 (FJMB80062_35730) 3839336..3841246 + 1911 WP_022649376.1 hypothetical protein -
NGK32_RS18345 (FJMB80062_35740) 3841243..3842937 + 1695 WP_022649377.1 hypothetical protein -
NGK32_RS18350 (FJMB80062_35750) 3843089..3843580 - 492 WP_022649378.1 GNAT family N-acetyltransferase Toxin
NGK32_RS18355 (FJMB80062_35760) 3843577..3843870 - 294 WP_022649379.1 DUF1778 domain-containing protein Antitoxin
NGK32_RS18360 (FJMB80062_35770) 3843972..3845765 - 1794 WP_022649380.1 hybrid sensor histidine kinase/response regulator -
NGK32_RS18365 (FJMB80062_35780) 3845762..3846394 - 633 WP_022649381.1 response regulator transcription factor -
NGK32_RS18370 (FJMB80062_35790) 3846570..3846884 + 315 WP_003862457.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3838369..3846884 8515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(83-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17469.36 Da        Isoelectric Point: 8.1405

>T41188 WP_022649378.1 NZ_AP025812:c3843580-3843089 [Enterobacter hormaechei]
MISAPVPLRAGHILTPFCCGVDSMDNWLKQRAMKNQASGASRTFVCCGGDSNVLAYYALASSAVTTNTVPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAESIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLV
ASI

Download         Length: 492 bp

>T41188 NZ_AP025812:c3843580-3843089 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10959.58 Da        Isoelectric Point: 8.6351

>AT41188 WP_022649379.1 NZ_AP025812:c3843870-3843577 [Enterobacter hormaechei]
MPAANTMAVKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLTRLDQAPAPN
AALRKTMQTLAPWEQEK

Download         Length: 294 bp

>AT41188 NZ_AP025812:c3843870-3843577 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A431SHU0

Antitoxin

Source ID Structure
AlphaFold DB A0A3S0FY21

References