Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1084319..1084940 Replicon chromosome
Accession NZ_AP025812
Organism Enterobacter hormaechei strain FUJ80062

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGK32_RS05165 Protein ID WP_015571250.1
Coordinates 1084319..1084537 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGK32_RS05170 Protein ID WP_006809850.1
Coordinates 1084566..1084940 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK32_RS05135 (FJMB80062_09920) 1080331..1080591 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGK32_RS05140 1080594..1080734 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGK32_RS05145 (FJMB80062_09930) 1080731..1081441 - 711 WP_022647267.1 GNAT family protein -
NGK32_RS05150 (FJMB80062_09940) 1081544..1083004 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
NGK32_RS05155 (FJMB80062_09950) 1082976..1083443 - 468 WP_022647269.1 YlaC family protein -
NGK32_RS05160 (FJMB80062_09960) 1083560..1084111 - 552 WP_022647270.1 maltose O-acetyltransferase -
NGK32_RS05165 (FJMB80062_09970) 1084319..1084537 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGK32_RS05170 (FJMB80062_09980) 1084566..1084940 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGK32_RS05175 (FJMB80062_09990) 1085450..1088596 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
NGK32_RS05180 (FJMB80062_10000) 1088619..1089812 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T41176 WP_015571250.1 NZ_AP025812:c1084537-1084319 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T41176 NZ_AP025812:c1084537-1084319 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT41176 WP_006809850.1 NZ_AP025812:c1084940-1084566 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT41176 NZ_AP025812:c1084940-1084566 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References