Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1081642..1082263 Replicon chromosome
Accession NZ_AP025804
Organism Enterobacter hormaechei strain FUJ80053

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGK45_RS05155 Protein ID WP_015571250.1
Coordinates 1081642..1081860 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGK45_RS05160 Protein ID WP_006809850.1
Coordinates 1081889..1082263 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK45_RS05125 (FJMB80053_09970) 1077654..1077914 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGK45_RS05130 1077917..1078057 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGK45_RS05135 (FJMB80053_09980) 1078054..1078764 - 711 WP_022647267.1 GNAT family protein -
NGK45_RS05140 (FJMB80053_09990) 1078867..1080327 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
NGK45_RS05145 (FJMB80053_10000) 1080299..1080766 - 468 WP_022647269.1 YlaC family protein -
NGK45_RS05150 (FJMB80053_10010) 1080883..1081434 - 552 WP_022647270.1 maltose O-acetyltransferase -
NGK45_RS05155 (FJMB80053_10020) 1081642..1081860 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGK45_RS05160 (FJMB80053_10030) 1081889..1082263 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGK45_RS05165 (FJMB80053_10040) 1082773..1085919 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
NGK45_RS05170 (FJMB80053_10050) 1085942..1087135 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T41128 WP_015571250.1 NZ_AP025804:c1081860-1081642 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T41128 NZ_AP025804:c1081860-1081642 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT41128 WP_006809850.1 NZ_AP025804:c1082263-1081889 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT41128 NZ_AP025804:c1082263-1081889 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References