Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1080249..1080870 Replicon chromosome
Accession NZ_AP025801
Organism Enterobacter hormaechei strain FUJ80024

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGJ01_RS05100 Protein ID WP_015571250.1
Coordinates 1080249..1080467 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGJ01_RS05105 Protein ID WP_006809850.1
Coordinates 1080496..1080870 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGJ01_RS05070 (FJMB80024_09900) 1076261..1076521 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGJ01_RS05075 1076524..1076664 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGJ01_RS05080 (FJMB80024_09910) 1076661..1077371 - 711 WP_022647267.1 GNAT family protein -
NGJ01_RS05085 (FJMB80024_09920) 1077474..1078934 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
NGJ01_RS05090 (FJMB80024_09930) 1078906..1079373 - 468 WP_022647269.1 YlaC family protein -
NGJ01_RS05095 (FJMB80024_09940) 1079490..1080041 - 552 WP_022647270.1 maltose O-acetyltransferase -
NGJ01_RS05100 (FJMB80024_09950) 1080249..1080467 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGJ01_RS05105 (FJMB80024_09960) 1080496..1080870 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGJ01_RS05110 (FJMB80024_09970) 1081380..1084526 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
NGJ01_RS05115 (FJMB80024_09980) 1084549..1085742 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T41112 WP_015571250.1 NZ_AP025801:c1080467-1080249 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T41112 NZ_AP025801:c1080467-1080249 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT41112 WP_006809850.1 NZ_AP025801:c1080870-1080496 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT41112 NZ_AP025801:c1080870-1080496 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References