Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3847392..3848173 Replicon chromosome
Accession NZ_AP025797
Organism Enterobacter hormaechei strain FUJ80022

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A431SHU0
Locus tag NGK25_RS18390 Protein ID WP_022649378.1
Coordinates 3847392..3847883 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A3S0FY21
Locus tag NGK25_RS18395 Protein ID WP_022649379.1
Coordinates 3847880..3848173 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK25_RS18375 (FJMB80022_36030) 3842672..3843124 - 453 WP_022649375.1 hypothetical protein -
NGK25_RS18380 (FJMB80022_36040) 3843639..3845549 + 1911 WP_022649376.1 hypothetical protein -
NGK25_RS18385 (FJMB80022_36050) 3845546..3847240 + 1695 WP_022649377.1 hypothetical protein -
NGK25_RS18390 (FJMB80022_36060) 3847392..3847883 - 492 WP_022649378.1 GNAT family N-acetyltransferase Toxin
NGK25_RS18395 (FJMB80022_36070) 3847880..3848173 - 294 WP_022649379.1 DUF1778 domain-containing protein Antitoxin
NGK25_RS18400 (FJMB80022_36080) 3848275..3850068 - 1794 WP_022649380.1 hybrid sensor histidine kinase/response regulator -
NGK25_RS18405 (FJMB80022_36090) 3850065..3850697 - 633 WP_022649381.1 response regulator transcription factor -
NGK25_RS18410 (FJMB80022_36100) 3850873..3851187 + 315 WP_003862457.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3842672..3851187 8515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(83-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17469.36 Da        Isoelectric Point: 8.1405

>T41092 WP_022649378.1 NZ_AP025797:c3847883-3847392 [Enterobacter hormaechei]
MISAPVPLRAGHILTPFCCGVDSMDNWLKQRAMKNQASGASRTFVCCGGDSNVLAYYALASSAVTTNTVPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAESIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLV
ASI

Download         Length: 492 bp

>T41092 NZ_AP025797:c3847883-3847392 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10959.58 Da        Isoelectric Point: 8.6351

>AT41092 WP_022649379.1 NZ_AP025797:c3848173-3847880 [Enterobacter hormaechei]
MPAANTMAVKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLTRLDQAPAPN
AALRKTMQTLAPWEQEK

Download         Length: 294 bp

>AT41092 NZ_AP025797:c3848173-3847880 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A431SHU0

Antitoxin

Source ID Structure
AlphaFold DB A0A3S0FY21

References