Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3486377..3487116 Replicon chromosome
Accession NZ_AP025797
Organism Enterobacter hormaechei strain FUJ80022

Toxin (Protein)


Gene name yeeV Uniprot ID A0A431SEP0
Locus tag NGK25_RS16635 Protein ID WP_022649088.1
Coordinates 3486736..3487116 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A431SER1
Locus tag NGK25_RS16630 Protein ID WP_022649087.1
Coordinates 3486377..3486703 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK25_RS16615 (FJMB80022_32610) 3482271..3484931 - 2661 WP_022649084.1 antiviral RADAR system adenosine triphosphatase RdrA -
NGK25_RS16620 (FJMB80022_32620) 3485561..3485884 + 324 WP_022649085.1 antirestriction protein -
NGK25_RS16625 (FJMB80022_32630) 3485899..3486366 + 468 WP_022649086.1 DNA repair protein RadC -
NGK25_RS16630 (FJMB80022_32640) 3486377..3486703 + 327 WP_022649087.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
NGK25_RS16635 (FJMB80022_32650) 3486736..3487116 + 381 WP_022649088.1 TA system toxin CbtA family protein Toxin
NGK25_RS16640 (FJMB80022_32660) 3487113..3487340 + 228 WP_022649089.1 DUF5983 family protein -
NGK25_RS16645 (FJMB80022_32670) 3487732..3488025 + 294 WP_022649090.1 contact-dependent growth inhibition system immunity protein -
NGK25_RS16650 (FJMB80022_32680) 3488369..3489685 - 1317 WP_022649091.1 amidase family protein -
NGK25_RS16655 (FJMB80022_32690) 3489740..3491035 - 1296 WP_022649092.1 histidinol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3463268..3488025 24757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-110)

Antitoxin

Antitoxin CbeA

(5-103)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13850.66 Da        Isoelectric Point: 5.8788

>T41090 WP_022649088.1 NZ_AP025797:3486736-3487116 [Enterobacter hormaechei]
MQTLPLPEPRAAEPCPSPVQVWQLLLTHLLDKHYGLTLNDTPFGDDGVIQAHINAGISLCDAVNFIVERYELLRTDHSSS
SLTEHSSLIGAIDILRAHRATGLIVGSGYSTITRITTGQYREDKTQ

Download         Length: 381 bp

>T41090 NZ_AP025797:3486736-3487116 [Enterobacter hormaechei]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11923.47 Da        Isoelectric Point: 5.8762

>AT41090 WP_022649087.1 NZ_AP025797:3486377-3486703 [Enterobacter hormaechei]
MHSEQWGLTCGKTPCFSAKLVQEGNRLHFLADRAEFSGDLSDDEALRLDQAFPVILKQLEQMLSAGELNPRHQHRVTLHL
NGLTCEADTLGSHGYLYIAIYPSAVATG

Download         Length: 327 bp

>AT41090 NZ_AP025797:3486377-3486703 [Enterobacter hormaechei]
ATGCATTCTGAACAATGGGGACTGACGTGCGGTAAAACGCCGTGCTTCAGTGCCAAGCTGGTGCAGGAAGGCAACCGGCT
GCACTTTCTAGCTGACCGAGCAGAGTTTAGTGGCGACTTGAGCGACGATGAGGCACTGCGCCTCGACCAGGCTTTTCCGG
TGATATTGAAGCAGCTGGAACAAATGCTTTCAGCCGGTGAGCTGAATCCCCGCCATCAGCATCGCGTCACTCTGCATCTT
AACGGGCTAACCTGCGAAGCCGACACGCTCGGTTCGCACGGCTACCTTTACATCGCGATTTATCCCTCAGCGGTAGCTAC
CGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A431SEP0

Antitoxin

Source ID Structure
AlphaFold DB A0A431SER1

References