Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2226622..2227361 Replicon chromosome
Accession NZ_AP025792
Organism Enterobacter hormaechei strain FUJ80017

Toxin (Protein)


Gene name tacT Uniprot ID A0A3S0FZ61
Locus tag NGK49_RS10610 Protein ID WP_022648141.1
Coordinates 2226622..2227107 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag NGK49_RS10615 Protein ID WP_003857131.1
Coordinates 2227095..2227361 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK49_RS10580 2222148..2223356 + 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
NGK49_RS10585 (FJMB80017_20770) 2223369..2223800 + 432 WP_251259014.1 alpha/beta hydrolase -
NGK49_RS10590 (FJMB80017_20780) 2223896..2224660 + 765 WP_022648136.1 SDR family oxidoreductase -
NGK49_RS10595 (FJMB80017_20800) 2225327..2225590 + 264 WP_022648138.1 DUF4225 domain-containing protein -
NGK49_RS10600 (FJMB80017_20810) 2225565..2225894 - 330 WP_022648139.1 hypothetical protein -
NGK49_RS10605 (FJMB80017_20820) 2226027..2226596 + 570 WP_022648140.1 hypothetical protein -
NGK49_RS10610 (FJMB80017_20830) 2226622..2227107 - 486 WP_022648141.1 GNAT family N-acetyltransferase Toxin
NGK49_RS10615 (FJMB80017_20840) 2227095..2227361 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
NGK49_RS10620 (FJMB80017_20850) 2227425..2228354 - 930 WP_022648142.1 LysR family transcriptional regulator -
NGK49_RS10625 (FJMB80017_20860) 2228484..2229872 + 1389 WP_022648143.1 MFS transporter -
NGK49_RS10630 (FJMB80017_20870) 2229851..2230408 - 558 WP_022648144.1 OmpH family outer membrane protein -
NGK49_RS10635 (FJMB80017_20880) 2230529..2231665 - 1137 WP_022648145.1 type 1 fimbrial protein -
NGK49_RS10640 (FJMB80017_20890) 2231689..2232267 - 579 WP_022648146.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2216808..2227361 10553
- flank IS/Tn - - 2222148..2223356 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17558.24 Da        Isoelectric Point: 9.6275

>T41049 WP_022648141.1 NZ_AP025792:c2227107-2226622 [Enterobacter hormaechei]
VGRVTPPEPLSSVHQLAEFISGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T41049 NZ_AP025792:c2227107-2226622 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT41049 WP_003857131.1 NZ_AP025792:c2227361-2227095 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT41049 NZ_AP025792:c2227361-2227095 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S0FZ61

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References