Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1080303..1080924 Replicon chromosome
Accession NZ_AP025792
Organism Enterobacter hormaechei strain FUJ80017

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGK49_RS05145 Protein ID WP_015571250.1
Coordinates 1080303..1080521 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGK49_RS05150 Protein ID WP_006809850.1
Coordinates 1080550..1080924 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK49_RS05115 (FJMB80017_10010) 1076315..1076575 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGK49_RS05120 1076578..1076718 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGK49_RS05125 (FJMB80017_10020) 1076715..1077425 - 711 WP_022647267.1 GNAT family protein -
NGK49_RS05130 (FJMB80017_10040) 1077528..1078988 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
NGK49_RS05135 (FJMB80017_10050) 1078960..1079427 - 468 WP_022647269.1 YlaC family protein -
NGK49_RS05140 (FJMB80017_10060) 1079544..1080095 - 552 WP_022647270.1 maltose O-acetyltransferase -
NGK49_RS05145 (FJMB80017_10070) 1080303..1080521 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGK49_RS05150 (FJMB80017_10080) 1080550..1080924 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGK49_RS05155 (FJMB80017_10090) 1081434..1084580 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
NGK49_RS05160 (FJMB80017_10100) 1084603..1085796 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T41048 WP_015571250.1 NZ_AP025792:c1080521-1080303 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T41048 NZ_AP025792:c1080521-1080303 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT41048 WP_006809850.1 NZ_AP025792:c1080924-1080550 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT41048 NZ_AP025792:c1080924-1080550 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References