Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1114065..1114686 Replicon chromosome
Accession NZ_AP025782
Organism Enterobacter hormaechei strain FUJ80011

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGK66_RS05290 Protein ID WP_015571250.1
Coordinates 1114065..1114283 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGK66_RS05295 Protein ID WP_006809850.1
Coordinates 1114312..1114686 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK66_RS05260 (FJMB80011_10220) 1110077..1110337 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGK66_RS05265 1110340..1110480 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGK66_RS05270 (FJMB80011_10230) 1110477..1111187 - 711 WP_022647267.1 GNAT family protein -
NGK66_RS05275 (FJMB80011_10240) 1111290..1112750 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
NGK66_RS05280 (FJMB80011_10250) 1112722..1113189 - 468 WP_022647269.1 YlaC family protein -
NGK66_RS05285 (FJMB80011_10260) 1113306..1113857 - 552 WP_022647270.1 maltose O-acetyltransferase -
NGK66_RS05290 (FJMB80011_10270) 1114065..1114283 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGK66_RS05295 (FJMB80011_10280) 1114312..1114686 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGK66_RS05300 (FJMB80011_10290) 1115196..1118342 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
NGK66_RS05305 (FJMB80011_10300) 1118365..1119558 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T40984 WP_015571250.1 NZ_AP025782:c1114283-1114065 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T40984 NZ_AP025782:c1114283-1114065 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT40984 WP_006809850.1 NZ_AP025782:c1114686-1114312 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT40984 NZ_AP025782:c1114686-1114312 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References