Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 118497..120120 Replicon plasmid pFJMB80008_1
Accession NZ_AP025781
Organism Enterobacter hormaechei strain FUJ80008

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag NGK30_RS23520 Protein ID WP_000088044.1
Coordinates 118795..120120 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag NGK30_RS23515 Protein ID WP_012477377.1
Coordinates 118497..118790 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK30_RS23495 (FJMB80008_46060) 114296..114688 + 393 WP_000341066.1 hypothetical protein -
NGK30_RS23500 (FJMB80008_46070) 115347..116003 + 657 WP_000462754.1 trypsin-like serine protease -
NGK30_RS23505 (FJMB80008_46090) 116206..116703 + 498 WP_087878322.1 hypothetical protein -
NGK30_RS23510 (FJMB80008_46100) 116708..118096 + 1389 WP_000083579.1 hypothetical protein -
NGK30_RS23515 (FJMB80008_46110) 118497..118790 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
NGK30_RS23520 (FJMB80008_46120) 118795..120120 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
NGK30_RS23525 (FJMB80008_46130) 120181..120387 + 207 WP_000134171.1 hypothetical protein -
NGK30_RS23530 (FJMB80008_46140) 120488..120898 + 411 WP_000985909.1 hypothetical protein -
NGK30_RS23535 (FJMB80008_46150) 120911..121726 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
NGK30_RS23540 (FJMB80008_46160) 121979..122404 + 426 WP_000278471.1 hypothetical protein -
NGK30_RS23545 (FJMB80008_46170) 122953..123261 + 309 WP_001224686.1 hypothetical protein -
NGK30_RS23550 (FJMB80008_46180) 123277..124134 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aac(6')-IIc / blaIMP-1 / qnrB6 / tet(B) - 1..366446 366446


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T40982 WP_000088044.1 NZ_AP025781:118795-120120 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T40982 NZ_AP025781:118795-120120 [Enterobacter hormaechei]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT40982 WP_012477377.1 NZ_AP025781:118497-118790 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT40982 NZ_AP025781:118497-118790 [Enterobacter hormaechei]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References