Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2297551..2298290 Replicon chromosome
Accession NZ_AP025780
Organism Enterobacter hormaechei strain FUJ80008

Toxin (Protein)


Gene name tacT Uniprot ID A0A3S0FZ61
Locus tag NGK30_RS11015 Protein ID WP_022648141.1
Coordinates 2297551..2298036 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag NGK30_RS11020 Protein ID WP_003857131.1
Coordinates 2298024..2298290 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGK30_RS10990 (FJMB80008_21540) 2293707..2294729 + 1023 WP_022648135.1 alpha/beta hydrolase -
NGK30_RS10995 (FJMB80008_21550) 2294825..2295589 + 765 WP_022648136.1 SDR family oxidoreductase -
NGK30_RS11000 (FJMB80008_21570) 2296256..2296519 + 264 WP_022648138.1 DUF4225 domain-containing protein -
NGK30_RS11005 (FJMB80008_21580) 2296494..2296823 - 330 WP_022648139.1 hypothetical protein -
NGK30_RS11010 (FJMB80008_21590) 2296956..2297525 + 570 WP_022648140.1 hypothetical protein -
NGK30_RS11015 (FJMB80008_21600) 2297551..2298036 - 486 WP_022648141.1 GNAT family N-acetyltransferase Toxin
NGK30_RS11020 (FJMB80008_21610) 2298024..2298290 - 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
NGK30_RS11025 (FJMB80008_21620) 2298354..2299283 - 930 WP_022648142.1 LysR family transcriptional regulator -
NGK30_RS11030 (FJMB80008_21630) 2299413..2300801 + 1389 WP_022648143.1 MFS transporter -
NGK30_RS11035 (FJMB80008_21640) 2300780..2301337 - 558 WP_022648144.1 OmpH family outer membrane protein -
NGK30_RS11040 (FJMB80008_21650) 2301458..2302594 - 1137 WP_022648145.1 type 1 fimbrial protein -
NGK30_RS11045 (FJMB80008_21660) 2302618..2303196 - 579 WP_022648146.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2274684..2298290 23606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17558.24 Da        Isoelectric Point: 9.6275

>T40969 WP_022648141.1 NZ_AP025780:c2298036-2297551 [Enterobacter hormaechei]
VGRVTPPEPLSSVHQLAEFISGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T40969 NZ_AP025780:c2298036-2297551 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT40969 WP_003857131.1 NZ_AP025780:c2298290-2298024 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT40969 NZ_AP025780:c2298290-2298024 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S0FZ61

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References