Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1084575..1085196 Replicon chromosome
Accession NZ_AP025770
Organism Enterobacter hormaechei strain FUJ80003

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag NGJ61_RS05165 Protein ID WP_015571250.1
Coordinates 1084575..1084793 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag NGJ61_RS05170 Protein ID WP_006809850.1
Coordinates 1084822..1085196 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NGJ61_RS05135 (FJMB80003_10010) 1080587..1080847 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
NGJ61_RS05140 1080850..1080990 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NGJ61_RS05145 (FJMB80003_10020) 1080987..1081697 - 711 WP_022647267.1 GNAT family protein -
NGJ61_RS05150 (FJMB80003_10040) 1081800..1083260 + 1461 WP_022647268.1 PLP-dependent aminotransferase family protein -
NGJ61_RS05155 (FJMB80003_10050) 1083232..1083699 - 468 WP_022647269.1 YlaC family protein -
NGJ61_RS05160 (FJMB80003_10060) 1083816..1084367 - 552 WP_022647270.1 maltose O-acetyltransferase -
NGJ61_RS05165 (FJMB80003_10070) 1084575..1084793 - 219 WP_015571250.1 HHA domain-containing protein Toxin
NGJ61_RS05170 (FJMB80003_10080) 1084822..1085196 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
NGJ61_RS05175 (FJMB80003_10090) 1085706..1088852 - 3147 WP_022647271.1 multidrug efflux RND transporter permease subunit AcrB -
NGJ61_RS05180 (FJMB80003_10100) 1088875..1090068 - 1194 WP_022647272.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T40905 WP_015571250.1 NZ_AP025770:c1084793-1084575 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T40905 NZ_AP025770:c1084793-1084575 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT40905 WP_006809850.1 NZ_AP025770:c1085196-1084822 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT40905 NZ_AP025770:c1085196-1084822 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References