Detailed information of TA system TA040867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 140292..140851
• Replicon: plasmid pla1
• Accession: NZ_AP025755
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0B1JMW5
• Locus tag: QUE34_RS27485
• Protein ID: WP_000421249.1
• Coordinates: 140292..140567 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0B1JM87
• Locus tag: QUE34_RS27490
• Protein ID: WP_001355337.1
• Coordinates: 140567..140851 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS27445 (135937)	135937..136400	-	464	Protein_169	DUF1380 family protein	-
QUE34_RS27450 (136360)	136360..136581	-	222	WP_001104895.1	hypothetical protein	-
QUE34_RS27455 (136582)	136582..137265	-	684	WP_000085877.1	DNA methylase	-
QUE34_RS27460 (137344)	137344..137640	-	297	WP_001364075.1	hypothetical protein	-
QUE34_RS27465 (137784)	137784..138746	+	963	WP_105279257.1	plasmid segregation protein ParM	-
QUE34_RS27470 (138749)	138749..139099	+	351	WP_024166183.1	plasmid partitioning/stability family protein	-
QUE34_RS27475 (139206)	139206..139565	+	360	WP_000765123.1	hypothetical protein	-
QUE34_RS27480 (139983)	139983..140186	-	204	Protein_176	hypothetical protein	-
QUE34_RS27485 (140292)	140292..140567	-	276	WP_000421249.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE34_RS27490 (140567)	140567..140851	-	285	WP_001355337.1	ribbon-helix-helix protein, CopG family	Antitoxin
QUE34_RS27495 (141190)	141190..141399	+	210	WP_157839880.1	hypothetical protein	-
QUE34_RS27500 (141572)	141572..141977	+	406	Protein_180	hypothetical protein	-
QUE34_RS27505 (141984)	141984..142205	+	222	Protein_181	integrase core domain-containing protein	-
QUE34_RS27510 (142351)	142351..142941	+	591	WP_001355339.1	hypothetical protein	-
QUE34_RS27515 (144626)	144626..145498	+	873	Protein_183	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..215429	215429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10722.42 Da
• Isoelectric Point: 9.9324

>T40867 WP_000421249.1 NZ_AP025755:c140567-140292 [Escherichia sp. KS167_9B]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T40867 NZ_AP025755:c140567-140292 [Escherichia sp. KS167_9B]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10550.99 Da
• Isoelectric Point: 5.6706

>AT40867 WP_001355337.1 NZ_AP025755:c140851-140567 [Escherichia sp. KS167_9B]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Nucleotide

• Length: 285 bp

>AT40867 NZ_AP025755:c140851-140567 [Escherichia sp. KS167_9B]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Structures

Toxin

• AlphaFold DB: A0A0B1JMW5

Antitoxin

• AlphaFold DB: A0A0B1JM87