Detailed information of TA system TA040863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 91621..92246
• Replicon: plasmid pla1
• Accession: NZ_AP025755
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUE34_RS27145
• Protein ID: WP_000911316.1
• Coordinates: 91848..92246 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: QUE34_RS27140
• Protein ID: WP_000450526.1
• Coordinates: 91621..91848 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS27140 (91621)	91621..91848	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUE34_RS27145 (91848)	91848..92246	+	399	WP_000911316.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUE34_RS27150 (92255)	92255..94399	-	2145	WP_000009375.1	type IV conjugative transfer system coupling protein TraD	-
QUE34_RS27155 (94455)	94455..95185	-	731	Protein_111	hypothetical protein	-
QUE34_RS27160 (95420)	95420..96031	-	612	WP_000908443.1	hypothetical protein	-
QUE34_RS27165 (96103)	96103..96834	-	732	WP_001364068.1	conjugal transfer complement resistance protein TraT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..215429	215429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14915.18 Da
• Isoelectric Point: 7.8605

>T40863 WP_000911316.1 NZ_AP025755:91848-92246 [Escherichia sp. KS167_9B]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T40863 NZ_AP025755:91848-92246 [Escherichia sp. KS167_9B]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCAGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGACCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT40863 WP_000450526.1 NZ_AP025755:91621-91848 [Escherichia sp. KS167_9B]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT40863 NZ_AP025755:91621-91848 [Escherichia sp. KS167_9B]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA