Detailed information of TA system TA040856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5250513..5251089
• Replicon: chromosome
• Accession: NZ_AP025754
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: QUE34_RS26005
• Protein ID: WP_103253473.1
• Coordinates: 5250513..5250800 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: QUE34_RS26010
• Protein ID: WP_000063156.1
• Coordinates: 5250787..5251089 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS25990 (5246113)	5246113..5247477	-	1365	WP_103253470.1	restriction endonuclease subunit S	-
QUE34_RS25995 (5247467)	5247467..5249086	-	1620	WP_103253471.1	class I SAM-dependent DNA methyltransferase	-
QUE34_RS26000 (5249150)	5249150..5250316	-	1167	WP_103253472.1	restriction endonuclease	-
QUE34_RS26005 (5250513)	5250513..5250800	+	288	WP_103253473.1	BrnT family toxin	Toxin
QUE34_RS26010 (5250787)	5250787..5251089	+	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
QUE34_RS26015 (5251123)	5251123..5252079	-	957	WP_001295525.1	GTPase	-
QUE34_RS26020 (5252090)	5252090..5252293	-	204	WP_000467859.1	YbdD/YjiX family protein	-
QUE34_RS26025 (5252343)	5252343..5254493	-	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
QUE34_RS26030 (5254761)	5254761..5255981	-	1221	WP_000873752.1	amino acid deaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11247.83 Da
• Isoelectric Point: 9.9462

>T40856 WP_103253473.1 NZ_AP025754:5250513-5250800 [Escherichia sp. KS167_9B]
MPMEFKWDANKAKGNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIR
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T40856 NZ_AP025754:5250513-5250800 [Escherichia sp. KS167_9B]
ATGCCAATGGAGTTTAAATGGGATGCAAACAAGGCCAAAGGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGA
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT40856 WP_000063156.1 NZ_AP025754:5250787-5251089 [Escherichia sp. KS167_9B]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT40856 NZ_AP025754:5250787-5251089 [Escherichia sp. KS167_9B]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAATAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829G8Z0