Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5038233..5038730 Replicon chromosome
Accession NZ_AP025754
Organism Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE34_RS25005 Protein ID WP_001173343.1
Coordinates 5038557..5038730 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE34_RS25000 Protein ID WP_000398619.1
Coordinates 5038233..5038529 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE34_RS24980 (5034962) 5034962..5035681 - 720 WP_000611290.1 two-component system response regulator DcuR -
QUE34_RS24985 (5035678) 5035678..5037309 - 1632 WP_001216477.1 sensor histidine kinase -
QUE34_RS24990 (5037490) 5037490..5037720 + 231 WP_032304336.1 4Fe-4S mono-cluster protein YjdI -
QUE34_RS24995 (5037732) 5037732..5038004 + 273 WP_000405650.1 GNAT family N-acetyltransferase -
QUE34_RS25000 (5038233) 5038233..5038529 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE34_RS25005 (5038557) 5038557..5038730 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE34_RS25010 (5038849) 5038849..5040366 - 1518 WP_001295074.1 lysine--tRNA ligase -
QUE34_RS25015 (5040603) 5040603..5042060 - 1458 WP_032304338.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40855 WP_001173343.1 NZ_AP025754:5038557-5038730 [Escherichia sp. KS167_9B]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40855 NZ_AP025754:5038557-5038730 [Escherichia sp. KS167_9B]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTGCTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT40855 WP_000398619.1 NZ_AP025754:5038233-5038529 [Escherichia sp. KS167_9B]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40855 NZ_AP025754:5038233-5038529 [Escherichia sp. KS167_9B]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References