Detailed information of TA system TA040854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4324500..4325112
• Replicon: chromosome
• Accession: NZ_AP025754
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: QUE34_RS21670
• Protein ID: WP_000833473.1
• Coordinates: 4324927..4325112 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: QUE34_RS21665
• Protein ID: WP_000499744.1
• Coordinates: 4324500..4324910 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS21640 (4320706)	4320706..4321446	-	741	WP_000906829.1	MipA/OmpV family protein	-
QUE34_RS21645 (4321570)	4321570..4322544	+	975	WP_105268600.1	LysR family transcriptional regulator	-
QUE34_RS21650 (4322541)	4322541..4323677	-	1137	WP_000364885.1	HlyD family secretion protein	-
QUE34_RS21655 (4323683)	4323683..4324006	-	324	WP_000478193.1	DUF3302 domain-containing protein	-
QUE34_RS21660 (4324189)	4324189..4324503	+	315	WP_001064341.1	hypothetical protein	-
QUE34_RS21665 (4324500)	4324500..4324910	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QUE34_RS21670 (4324927)	4324927..4325112	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
QUE34_RS21675 (4325390)	4325390..4326928	-	1539	WP_000183976.1	aldehyde dehydrogenase AldB	-
QUE34_RS21680 (4327129)	4327129..4328280	-	1152	WP_000741504.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T40854 WP_000833473.1 NZ_AP025754:c4325112-4324927 [Escherichia sp. KS167_9B]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T40854 NZ_AP025754:c4325112-4324927 [Escherichia sp. KS167_9B]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGTAGTCATCATCAGTT
CCGCCACCCTGTCCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT40854 WP_000499744.1 NZ_AP025754:c4324910-4324500 [Escherichia sp. KS167_9B]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT40854 NZ_AP025754:c4324910-4324500 [Escherichia sp. KS167_9B]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9