Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3374265..3374848 Replicon chromosome
Accession NZ_AP025754
Organism Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag QUE34_RS16950 Protein ID WP_000254750.1
Coordinates 3374265..3374600 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag QUE34_RS16955 Protein ID WP_000581937.1
Coordinates 3374600..3374848 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE34_RS16935 (3370153) 3370153..3371451 - 1299 WP_000036723.1 phosphopyruvate hydratase -
QUE34_RS16940 (3371538) 3371538..3373175 - 1638 WP_286338862.1 CTP synthase (glutamine hydrolyzing) -
QUE34_RS16945 (3373403) 3373403..3374194 - 792 WP_032303686.1 nucleoside triphosphate pyrophosphohydrolase -
QUE34_RS16950 (3374265) 3374265..3374600 - 336 WP_000254750.1 endoribonuclease MazF Toxin
QUE34_RS16955 (3374600) 3374600..3374848 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
QUE34_RS16960 (3374926) 3374926..3377160 - 2235 WP_000226815.1 GTP pyrophosphokinase -
QUE34_RS16965 (3377208) 3377208..3378509 - 1302 WP_032303687.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T40850 WP_000254750.1 NZ_AP025754:c3374600-3374265 [Escherichia sp. KS167_9B]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T40850 NZ_AP025754:c3374600-3374265 [Escherichia sp. KS167_9B]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGTGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT40850 WP_000581937.1 NZ_AP025754:c3374848-3374600 [Escherichia sp. KS167_9B]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT40850 NZ_AP025754:c3374848-3374600 [Escherichia sp. KS167_9B]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTAGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References